Electrophoretic Properties of Sodium Dodecyl Sulfate and Related Changes in Its Concentration in SDS-polyacrylamide Gel Electrophoresis

Abstract

Sodium dodecyl sulfate (SDS) in a protein sample solution migrates in SDS-polyacryl-amide gel electrophoresis as a band with a mobility higher than those of protein bands. Behind this band, which is mostly composed of SDS micelles, SDS concentration is raised uniformly in a gel column as a result of the retardation effect of the gel matrix on SDS micelles. Electrophoretic patterns of SDS were obtained when SDS was omitted from various portions of the gel electrophoretic system.