Responses of fertile and sterile screwworm (Diptera: Calliphoridae) flies to bovine blood inoculated with bacteria originating from screwworm-infested animal wounds.
- 1 January 2002
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Medical Entomology
- Vol. 39 (1), 130-134
- https://doi.org/10.1603/0022-2585-39.1.130
Abstract
A simple bioassay system was developed to study locomotory and ovipositional responses of screwworm, Cochliomyia hominivorax (Coquerel), flies to bovine blood inoculated with eight species of coliform bacteria that were isolated from screwworm-infested animal wounds. When exposed to odors from bacteria-inoculated blood which was incubated for 72 h at 37°C, ≈50% of 7- and 10-d-old gravid females landed on the blood by the end of 15 min test exposure. Only 17% of 7-d-old reproductively sterile females (from irradiated pupae) with previtellogenic ovaries and 2% of 4-d-old vitellogenic females responded to the same treatment. Females generally reacted in greatest numbers to bacteria-inoculated blood incubated for 72 h, followed by 48 h, then 24 and 96 h. Males of all ages tested were unresponsive. Although oviposition occurred in tests with gravid females lasting for 1 h, with both inoculated blood and an uninoculated control, the inoculated sample was significantly better than the control at 48, 72, and 96 h incubation duration. Our results are consistent with the conclusion that the inoculated blood, when incubated for 48–72 h, gives off volatile chemicals which attract gravid females and contains an oviposition stimulant that acts following contact and feeding. The volatiles, once isolated and identified, may be useful for sampling gravid females in the field as well as improving the oviposition system in the mass-production facility of the screwworm eradication program.Keywords
This publication has 14 references indexed in Scilit:
- A New Starch-Grafted Gelling Agent for Screwworm (Diptera: Calliphoridae) Larval DietJournal of Economic Entomology, 1999
- Oviposition by Screwworm Flies (Diptera: Calliphoridae) on Contact with Host FluidsJournal of Economic Entomology, 1991
- Protein Feeding and Oviposition Effects on Attraction of Screwworm Flies (Diptera: Calliphoridae) to Host FluidsAnnals of the Entomological Society of America, 1990
- Multicomponent attractant for female screwworm flies,Cochliomyia hominivorax, in bovine bloodJournal of Chemical Ecology, 1989
- Reproductive Factors Affecting Responses of the Screwworm Fly, Cochliomyia hominivorax (Diptera: Calliphoridae), to an Attractant of Bacterial OriginAnnals of the Entomological Society of America, 1987
- Responses of gravid screwworm flies,Cochliomyia hominivorax, to whole wounds, wound fluid, and a standard blood attractant in olfactometer testsJournal of Chemical Ecology, 1983
- Effects of Cattle Dung on the Behavior and Survival of Adult Screwworms 1 and on the Development of their EggsEnvironmental Entomology, 1982
- Attraction and Ovipositional Response of Screwworms, Cochliomyia Hominivorax (Diptera: Calliphoridae), to Simulated Bovine Wounds1Journal of Medical Entomology, 1979
- Response of Three Colonies of Screwworm Flies to Proteins and Sugars in a Multiple-Choice OlfactometerJournal of Economic Entomology, 1971
- Olfactory Responses of the Adult Screw-worm After Removal of the Antennae, Mouthparts, Tarsi, and Legs1Journal of Economic Entomology, 1970