Approximately 1 kilobase of sequence 5' to the two myosin light-chain 1f/3f gene cap sites is sufficient for differentiation-dependent expression.
Open Access
- 1 March 1988
- journal article
- research article
- Published by Taylor & Francis in Molecular and Cellular Biology
- Vol. 8 (3), 1361-1365
- https://doi.org/10.1128/mcb.8.3.1361
Abstract
Approximately 1 kilobase of genomic DNA from the chicken fast myosin light-chain 1f/3f gene 5' to the transcriptional start sites for each light-chain mRNA was sufficient for differentiation-dependent, tissue-restricted expression. This was determined in primary chick myoblast cultures transfected with the chloramphenicol acetyltransferase (CAT) expression vector p8CAT containing these 5'-flanking sequences. The expression of CAT activity from both light-chain promoters was 10- to 20-fold higher in differentiated myotubes than in fibroblasts or myoblasts grown in bromodeoxyuridine. In contrast, the beta-actin and Rous sarcoma virus promoters joined to the CAT gene were expressed equally in all cell backgrounds tested. Even though the relative timing of light-chain 1f and 3f expression was altered, tissue-restricted, differentiation-dependent expression of the light-chain mRNAs was maintained with these 5' cis-acting sequence elements.This publication has 17 references indexed in Scilit:
- Transfection of a DNA locus that mediates the conversion of 10T12 fibroblasts to myoblastsCell, 1986
- Plasticity of the Differentiated StateScience, 1985
- A single locus in the mouse encodes both myosin light chains 1 and 3, a second locus corresponds to a related pseudogeneCell, 1984
- Regulation of rat myosin light-chain synthesis in heterokaryons between 5-bromodeoxyuridine-blocked rat myoblasts and differentiated chick myocytes.The Journal of cell biology, 1983
- Myosin light-chain expression during avian muscle developmentThe Journal of cell biology, 1983
- Changes in the mRNA population of chick myoblasts during myogenesis in vitroCell, 1977
- 5-Bromodeoxyuridine inhibition of differentiation. Kinetics of inhibition and reversal in myoblastsDevelopmental Biology, 1974
- Myosin synthesis in cultures of differentiating chicken embryo skeletal muscleDevelopmental Biology, 1972
- A KINETIC ANALYSIS OF MYOGENESIS IN VITROThe Journal of cell biology, 1972
- INHIBITION OF MYOBLAST FUSION AFTER ONE ROUND OF DNA SYNTHESIS IN 5-BROMODEOXYURIDINEThe Journal of cell biology, 1970