Karyotype analysis in rice. 1. A new method for identifying all chromosome pairs.

Abstract

Mitotic chromosomes of rice, Oryza sativa L. (2n = 24), were identified by a new technique of cell suspending and flame-drying in which the chromosomes are well flattened and spread. Tissue treatment with KCl and enzyme caused segmental differentiation in chromosome stainability with Giemsa. All chromosomes were identified in a single prometaphase nucleus by individual characteristics. Twelve pairs of chromosomes differed in length and consisted of 5 metacentrics, 5 sub-metacentrics and 2 sub-telocentrics including a satellited pair.