Macrophage colony-stimulating factor purified from normal human urine Amino-terminal sequence and amino acid composition
- 5 October 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 222 (2), 341-344
- https://doi.org/10.1016/0014-5793(87)80398-8
Abstract
A macrophage colony-stimulating factor (M-CSF) was purified to homogeneity from a large amount of normal human urine. Microanalysis of the N-terminal amino acid sequence up to residue 44 revealed only a single residue difference from that deduced by other workers from the nucleotide sequence of M-CSF cDNA clones. The amino acid composition of the present preparation suggested that the M-CSF which we purified possessed a structure fitting the sequence 1-190 of TPA30-1 cell M-CSF deduced by Wong et al. [(1987) Science 235, 1504-1508].Keywords
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