ISOLATION AND IDENTIFICATION OF 17 -OESTRADIOL IN THE BILE OF A PREGNANT COW
- 1 April 1965
- journal article
- research article
- Published by Bioscientifica in Reproduction
- Vol. 9 (2), 137-147
- https://doi.org/10.1530/jrf.0.0090137
Abstract
Free 17[alpha]-estradiol has been isolated and identified in a pregnant cow''s bile. The identification was based on several specific color reactions, the behavior of different derivatives of the compound in alumina and thin-layer chromatography and finally on a great number of gas chromatographic investigations. A sensitive and highly specific color test for 17[alpha]-estradiol and its derivatives in thin-layer chromatograms, making use of phosphomoly-bdic acid, has been presented.Keywords
This publication has 7 references indexed in Scilit:
- Gas-chromatographic studies on oestrogens in the bile of pregnant womenBiochimica et Biophysica Acta (BBA) - General Subjects, 1965
- STOFFWECHSEL VON OESTRON, OESTRADIOL-17-ALPHA UND OESTRADIOL-17-BETA IN DER KANINCHENLEBER1960
- STUDIES ON OESTROGENS IN CATTLEActa Endocrinologica, 1958
- ON THE CHROMATOGRAPHIC ISOLATION OF TWO DIFFERENT KOBER CHROMOGENS FROM PREGNANT COW'S URINE, WITH SOME REMARKS ON THE ANALYTICAL PROCEDUREActa Endocrinologica, 1958
- Steroids and other lipids of pregnant goat's urineBiochemical Journal, 1957
- A chemical method for the determination of oestriol, oestrone and oestradiol in human urineBiochemical Journal, 1955
- Detection of steroids in paper chromatographyArchives of Biochemistry and Biophysics, 1952