Inhibition of herpes simplex virus-induced DNA polymerase activity and viral DNA replication by 9-(2-hydroxyethoxymethyl)guanine and its triphosphate
- 1 October 1979
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 32 (1), 72-77
- https://doi.org/10.1128/jvi.32.1.72-77.1979
Abstract
The effect of the nucleoside analog 9-(2-hydroxyethoxymethyl)guanine (acycloguanosine) on herpes simplex virus type 1 DNA synthesis was examined. Acycloguanosine inhibited herpesvirus DNA synthesis in virus-infected cells [human cervical carcinoma HeLa, African green monkey kidney Vero]. The synthesis of host cell DNA was only partially inhibited in actively growing cells at acycloguanosine concentrations several hundred-fold greater than the 50% effective dose for herpes simplex virus type 1. Studies using partially purified enzymes revealed that the triphosphate of this compound inhibited the virus-induced DNA polymerases (DNA nucleotidyltransferases) to a greater degree than the .alpha.-DNA polymerase of the host cell; that the inhibition was dependent upon the base composition of the template and that the triphosphate was a better substrate for the virus-induced polymerases than for the .alpha.-cellular DNA polymerases.This publication has 16 references indexed in Scilit:
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