Cytochemical Studies of Milk-Adapted Murine Lymphoma Cells (Strain P388 D1) Infected With Polyoma Virus

Abstract

Some aspects of the cytochemistry of milk-adapted murine lymphoma cells are presented. Infection of the cells with polyoma virus produces an alteration in the ribonucleoprotein (RNP) of the parachromatin of the nucleolus and nucleoplasm, and enlargement of the nucleus, nucleolus, and cytoplasm; vacuoles containing an abnormal RNP appear in the nucleus. Cell degeneration is completed by nuclear and cytoplasmic shrinkage, disappearance of cytoplasmic RNP, and karyorrhexis. It is suggested that the mechanisms of synthesis of normal RNP in the nucleus may be diverted to the production of RNP that is related to viral synthesis.