Polarographic Determination of Subnanogram Quantities of Free Platinum in Reaction Mixture with Dna
- 1 August 1990
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 23 (8), 1505-1518
- https://doi.org/10.1080/00032719008052504
Abstract
A simple and rapid differential pulse polarographic assay for measurement of platinum drug binding to DNA is described. The method makes it possible to determine the free (unbound) platinum compound in the presence of DNA or platinum - DNA complex. The method is based on the polarographic catalytic hydrogen current yielded by platinum (II) or (IV) complexes in formaldehyde - hydrazine - sulphuric acid background electrolyte, in which DNA or platinum - DNA complex precipitate. The lower level of analytical utility of this method is c. 1 × 10−9 M.Keywords
This publication has 16 references indexed in Scilit:
- Redox behaviour of anti-tumor platinum (II) compounds (carboplatin) at solid electrodesJournal of Pharmaceutical and Biomedical Analysis, 1987
- Differential pulse polarographic determination of trace levels of platinumAnalytical Chemistry, 1986
- Platinum determination in cis-dichlorodiammineplatinum(II)-DNA complexes by differential pulse polarographyAnalytical Biochemistry, 1984
- Polarographic activity of the antitumor drug cis-dichlorodiammineplatinum (II). The effect of hydrolysis and trans-isomerization of the drugCellular and Molecular Life Sciences, 1984
- Assessment of cisplatin reactivity with peptides and proteins using reverse-phase high-performance liquid chromatography and flameless atomic absorption spectroscopyAnalytical Biochemistry, 1982
- Kinetic analysis of the in vitro binding of radioactive cis- and trans-dichlorodiammineplatinum(II) to DNAChemico-Biological Interactions, 1980
- Platinum determination in DNA-platinum complexes by fluorescence spectrophotometryAnalytical Biochemistry, 1978
- DNA-platinum interactions in vitro with trans- and cis- Pt(NH3)2Cl2Bioinorganic Chemistry, 1975
- Spécificité de l'interaction DNA‐platine dosage du platine, pH métrieBiopolymers, 1975
- The influence of salts andpH on polarographic currents produced by denatured DNARadiation and Environmental Biophysics, 1970