Proteolipid and Calculus Matrix Calcification In Vitro

Abstract
The initiator of calculus matrix calcification, in vitro, was isolated. Crude phospholipid, known to contain the factor, was separated into five fractions by column chromatography. A single protein-containing fraction induced apatite formation during incubation in a metastable calcium phosphate solution. The nucleating fraction was identified as a proteolipid.

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