Quantification of Myosin Heavy‐Chain mRNA during Myogenesis
- 31 December 1977
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 82 (2), 601-608
- https://doi.org/10.1111/j.1432-1033.1978.tb12056.x
Abstract
Complementary DNA (cDNA) was synthesized from a 26-S poly(A)-containing RNA fraction consisting predominately of myosin mRNA and used to quantitative the amounts of myosin mRNA during development. RNA .cntdot. cDNAmyosin hybridizations were performed with RNA isolated from tertiary cultures of 10 day old chick embryo fibroblasts and the proportion of myosin RNA determined. The levels of myosin RNA present in primary 11 day old chick embryo myoblast cultures undergoing development were also analyzed by RNA .cntdot. cDNAmyosin hybridizations. Correlation of these values with the rate of myosin H chain synthesis indicated that myosin mRNA was subject to post-transcriptional or translational control.This publication has 39 references indexed in Scilit:
- Cytoplasmic distribution of pulse-labelled poly(A)-containing RNA, particularly 26 S RNA, during myoblast growth and differentiationJournal of Molecular Biology, 1976
- Preparation and characterization of myosin copy DNABiochemical and Biophysical Research Communications, 1976
- A mechanical dissociation method for preparation of muscle cell culturesJournal of Cellular Physiology, 1975
- Activation of myosin synthesis in fusing and mononucleated myoblastsJournal of Molecular Biology, 1975
- Synthesis of DNA complementary to cellular slime mold messenger RNA by reverse transcriptaseBiochemistry, 1974
- Kinetic studies of gene frequency: II. Complexity of globin complementary DNA and its hybridization characteristicsJournal of Molecular Biology, 1974
- Myosin synthesis in cultures of differentiating chicken embryo skeletal muscleDevelopmental Biology, 1972
- Kinetic complexity of RNA moleculesJournal of Molecular Biology, 1972
- Muscle differentiation and macromolecular synthesisJournal of Cellular Physiology, 1968
- Some Aspects of Myogenesis in VitroCirculation, 1961