A Method for Detection of Leukocyte Injury Based on Release of a Lysozyme-like Enzyme.

Abstract

A method is descr. for the detection of cell injury by the measurement of intracellular substances released upon injury. The cells studied have been human leukocytes, and the intracellular substance selected for measurement has been an enzyme with the properties of lysozyme. Application of the method is illustrated by data concerning the adverse effect of hypotonicity and of an antiserum to human leukocytes on the cells. In further studies beyond the scope of the present report, the method has been found to be useful in screening a variety of physical, chemical and biologic agents for adverse effect on the metabolism of human leukocytes, as well as for detecting reversal of such injurious effects by protective agents.