DETERMINATION OF SUCCINIC DEHYDROGENASE ACTIVITY IN TISSUE CULTURE
Open Access
- 1 March 1959
- journal article
- research article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 7 (2), 144-146
- https://doi.org/10.1177/7.2.144
Abstract
Two methods of demonstrating succinic dehydrogenase activity in tissue cultures of HeLa cells have been demonstrated. One method employs a protective layer of agar applied to the cells prior to incubation in the selected substrate. The other method uses a mixture of agar and substrate which is placed on the cells; incubation follows without the further addition of substrate.Keywords
This publication has 4 references indexed in Scilit:
- DEHYDROGENASE ACTIVITY OF SHORT-TERM TISSUE CULTURES1957
- Histochemical Demonstration of Succinic Dehydrogenase in Ascites Tumor CellsStain Technology, 1957
- HISTOCHEMICAL LOCALIZATION OF SPECIFIC OXIDATIVE ENZYMES III. EVALUATION STUDIES OF TETRAZOLIUM STAINING METHODS FOR DIPHOSPHOPYRIDINE NUCLEOTIDE DIAPHORASE, TRIPHOSPHOPYRIDINE NUCLEOTIDE DIAPHORASE AND THE SUCCINDEHYDROGENASE SYSTEMJournal of Histochemistry & Cytochemistry, 1956
- On the usefulness of tetrazolium salts as histochemical indicators of dehydrogenase activityThe Anatomical Record, 1952