Cyclic adenosine monophosphate and the development of polysphondylium
Open Access
- 1 October 1976
- journal article
- research article
- Published by The Company of Biologists in Journal of Cell Science
- Vol. 22 (1), 41-47
- https://doi.org/10.1242/jcs.22.1.41
Abstract
Centre formation in Polysphondylium violaceum is delayed for 2 h on buffered agar containing 10−3 M c-AMP, and for up to 22 h on unbuffered agar with the same c-AMP concentration. With ambient c-AMP concentrations as low as 10−8 M, P. pallidum forms numerous, small, atypical aggregates which do not fruit. This effect is independent of whether the agar is buffered. P. violaceum amoebae are weakly attracted to the tip of a microelectrode containing 10−3 or 10−4 M c-AMP, but the electrode cannot compete when natural centres form nearby. P. pallidum amoebae are not attracted. Aggregates of P. violaceum and of P. pallidum are strongly attracted to a microelectrode releasing c-AMP. The observation of Shaffer that Polysphondylium grex switch over from secreting an acrasin that attracts homologous amoebae to one that attracts the larger Dictyo- stelium species suggests that the second acrasin might be c-AMP. The above results strengthen this conjecture. As c-AMP inhibits centre formation, the secretion of c-AMP by older aggregates may explain an inhibition of centre formation in the ‘ overlay’ experiments of Shaffer.This publication has 14 references indexed in Scilit:
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