Viral alkaline nuclease in intranuclear dense bodies induced by herpes simplex infection
- 1 January 1986
- journal article
- research article
- Published by Wiley in Biology of the Cell
- Vol. 58 (1), 15-22
- https://doi.org/10.1111/j.1768-322x.1986.tb00485.x
Abstract
The distribution of the herpes simplex virus alkaline nuclease (HSV D Nase) in rabbit fibroblast cells infected with HSV type 1 or 2 (HSV-1 or HSV-2) has been examined with the aid of immunocytochemical techniques using gold particles as markers. HSV D Nase was found to be accumulated within infected nuclei as early as 2.5 hr post-infection. Labeled antibody to HSV D Nase subsequently increased in all nuclei after 7 hr and 17 hr. At all times post-infection the virus induced nuclear dense bodies were always the most intensely labeled structures. The association of HSV D Nase with nucleoprotein containing structures was readily dissociated by hypotonic shock and detergent treatment, but its association with the dense bodies was not disrupted. Nucleolar 100 kDa protein was found to be simultaneously present in the dense bodies. This suggests that HSV D Nase might interfere with ribosomal RNA synthesis and play a role in the degradation of the host-cell metabolism.This publication has 19 references indexed in Scilit:
- Mechanism of Degradation of Duplex DNA by the DNase Induced by Herpes Simplex VirusJournal of Virology, 1981
- Enhancement of structural preservation and immunocytochemical staining in low temperature embedded pancreatic tissue.Journal of Histochemistry & Cytochemistry, 1981
- Alkaline deoxyribonuclease induced by herpes simplex virus type 1: composition and properties of the purified enzymeVirology, 1980
- Differences in the Morphology of Herpes Simplex Virus Infected Cells: I. Comparative Scanning and Transmission Electron Microscopic Studies on HSV-1 Infected HEp-2 and Chick Embryo Fibroblast CellsJournal of General Virology, 1979
- Quantitative correlation of morphological alterations of the nucleus with functional events during in vitro infection of glial cells with herpes simplex hominis (HSV 2)Journal of Ultrastructure Research, 1978
- Visualization of two different types of nuclear transcriptional complexes in rat liver cellsJournal of Ultrastructure Research, 1977
- Herpesvirus EnvelopmentJournal of Virology, 1968
- Normal and 5-bromodeoxyuridine-inhibited development of herpes simplex virusExperimental Cell Research, 1966
- Relationship Between the Envelope and the Infectivity of Herpes Simplex Virus.Experimental Biology and Medicine, 1964
- Virus isolated from the brain of a patient with multiple sclerosisExperimental Neurology, 1964