ISOLATION OF OVINE LYMPHOCYTES, GRANULOCYTES, AND MONOCYTES BY COUNTERFLOW CENTRIFUGATION ELUTRIATION
- 1 January 1982
- journal article
- research article
- Vol. 43 (8), 1467-1470
Abstract
A simple, brief 1-step counterflow centrifugation elutriation technique is described for the recovery of highly purified populations of lymphocytes and granulocytes from adult and fetal ovine peripheral blood. Monocytes are obtained as an enriched population. Lymphocyte and granulocyte recoveries average 80%, higher than with routine gradient centrifugation procedures. Inexpensive medium is used and it is completed in .apprx. 90 min. The individual blood cellular populations, when tested appropriately, demonstrate normal functional responses.This publication has 6 references indexed in Scilit:
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