Oligodendrocytes produce low molecular weight glycoproteins containing N-acetyl-D-glucosamine in their Golgi apparatus
- 1 March 1994
- Vol. 10 (3), 193-201
- https://doi.org/10.1002/glia.440100306
Abstract
Lectin histochemistry using the Griffonia simplicifolia II lectin (GSL II) has revealed a novel group of glycoproteins containing terminal N‐acetyl‐D‐glucosamine (GlcNAc) residues in oligodendrocytes. The GlcNAc‐containing glycoproteins were not present in other types of glial cells, but were expressed by some neuronal cell populations. Within oligodendrocytes their localization was confined to the Golgi apparatus, as determined ultrastructurally. Biochemical analyses using tricine/SDS‐polyacrylamide gel electrophoresis and western blotting with GSL II showed the GlcNAc‐containing glycoproteins to be insoluble, with molecular masses ranging from 15 to 30 kDa. Our study provides a first account of insoluble, GlcNAc‐rich 15–30 kDa glycoproteins in oligodendroglia. The findings are discussed in the context of the functional significance of other known oligodendrocyte glycoproteins.Keywords
This publication has 29 references indexed in Scilit:
- Postnatal development and localization of an N‐acetylgalactosamine containing glycoconjugate associated with nonpyramidal neurons in cat visual cortexJournal of Comparative Neurology, 1993
- Presence of an α-galactolipid on the cell surfaces of endothelial cells of human kidneyJournal of Molecular Histology, 1989
- Evidence for an early role for myelin‐associated glycoprotein in the process of myelinationGlia, 1989
- Oligodendrocytes but not astrocytes express apolipoprotein E after injury of rat optic nerveGlia, 1989
- Myelin basic protein and transferrin characterize different subpopulations of oligodendrocytes in rat primary glial culturesJournal of Neuroscience Research, 1988
- Glial and glycoconjugate boundaries during postnatal development of the central nervous systemDevelopmental Brain Research, 1987
- Lectin binding by resting and reactive microgliaJournal of Neurocytology, 1987
- Evidence for glycoconjugate in nociceptive primary sensory neurons and its origin from the golgi complexBrain Research, 1986
- A Specific 37,000-Dalton Protein That Accumulates in Regenerating but Not in Nonregenerating Mammalian NervesScience, 1985
- Myelin-Associated Glycoprotein in Development and DiseaseDevelopmental Neuroscience, 1983