CLASSIFICATION OF MICROORGANISMS BY ANALYSIS OF CHEMICAL COMPOSITION I
- 1 May 1963
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 85 (5), 1039-1044
- https://doi.org/10.1128/jb.85.5.1039-1044.1963
Abstract
Abel, K. (Melpar, Inc., Falls Church, Va.), H. deSchmertzing, and J. I. Peterson. Classification of microorganisms by analysis of chemical composition. I. Feasibility of utilizing gas chromatography. J. Bacteriol. 85:1039–1044. 1963.—The feasibility of utilizing gas chromatography as a sensitive and rapid method for the analysis of lipids as a natural basis for the classification of microorganisms by chemical composition was investigated. The lipids were extracted and transesterified to component carboxylic acid methyl esters in a single step, after which the methyl esters were resolved by gas chromatography to provide distinctive chromatographic elution patterns. Similarities in the lipid carboxylic acid distribution were noted among selected species of the family Enterobacteriaceae, and significant differences were noted among selected families of the class Schizomycetes.Keywords
This publication has 3 references indexed in Scilit:
- Ionization Methods for the Analysis of Gases and VaporsAnalytical Chemistry, 1961
- The Classification of Actinomycetes in Relation to Their Antibiotic ActivityAdvances in applied microbiology, 1961
- Gas-liquid partition chromatography: the separation and micro-estimation of volatile fatty acids from formic acid to dodecanoic acidBiochemical Journal, 1952