SOLID‐PHASE ENZYME IMMUNOASSAY OF IgM‐CLASS RHEUMATOID FACTOR: COMPARISON OF THREE METHODS FOR PREPARATION OF THE SOLID‐PHASE TARGET IgG
- 1 November 1980
- journal article
- research article
- Published by Wiley in Acta Pathologica Microbiologica Scandinavica Section C Immunology
- Vol. 88C (1-6), 127-130
- https://doi.org/10.1111/j.1699-0463.1980.tb00084.x
Abstract
Three solid-phase forms of human Ig[immunoglobulin]G were compared for their ability to function as the binding target in an enzyme immunoassay for IgM-class rheumatoid factor (RF). IgG was directly adsorbed to polystyrene beads (method A), or immunologically (method B) or covalently (method C) bound to protein adsorbed to beads. C is the method of choice for the preparation of the RF assay solid-phase IgG.Keywords
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