Denaturation and renaturation of Penicillium chrysogenum mycophage double-stranded ribonucleic acid in tetraalkylammonium salt solutions
- 1 May 1981
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 20 (11), 2999-3002
- https://doi.org/10.1021/bi00514a002
Abstract
The base composition dependence of double-stranded RNA melting was studied by observing the structure and widths of melting transitions for P. chrysogenum mycophage RNA and differences in melting temperatures of 2 RNA of different base composition. Double-stranded RNA melting is independent of base compositions in 3.5 M Et4NCl (tetraethylammonium chloride) and 4.6 M Me4NCl (tetramethylammonium chloride), where the melting temperatures are 25 and 92.degree. C, respectively. Double-stranded RNA renaturation rate constants are reported in Et4NCl solutions. The nucleation rate constant is .apprx. 10 times lower than that for double-stranded DNA. Each of the 3 similar, but separable RNA segments of P. chrysogenum mycophage is apparently unique.This publication has 7 references indexed in Scilit:
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