Superoxide production from nonenzymatically glycated protein
- 29 August 1988
- journal article
- Published by Wiley in FEBS Letters
- Vol. 236 (2), 406-410
- https://doi.org/10.1016/0014-5793(88)80066-8
Abstract
Nonenzymatically glycated human serum albumin and glycated poly‐lysine(Lys) in vitro brought about the reduction of nitroblue tetrazolium and ferricytochrome c at pH 9.06 and pH 7.8, respectively. This reduction was inhibited partially by superoxide dismutase (SOD). Glycated poly‐Lys caused the oxidation of NADH in the presence of LDH at pH 7.0 which was completely inhibited by SOD. Glycated material was found to function both as a reductant and an oxidant. The reactivity of glycated material is discussed and a possible mechanism by which superoxide is produced is proposed. Results may give a clue to diabetic complications.Keywords
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