The Location of DNA in RecA-DNA Helical Filaments
- 28 July 1989
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 245 (4916), 404-407
- https://doi.org/10.1126/science.2667137
Abstract
The helical filament that the RecA protein of Escherichia coli forms around DNA is the active apparatus in protein-catalyzed homologous genetic recombination. The actual position of DNA within this complex has been unknown. Image analysis has been performed on electron micrographs of filaments of RecA on double-stranded DNA and on single-stranded DNA to visualize a difference that is consistent with one strand of the double-stranded DNA. This localization of the DNA gives additional information about the unusual structure of DNA in the complex with RecA protein.Keywords
This publication has 20 references indexed in Scilit:
- Binding stoichiometry and structure of RecA-DNA complexes studied by flow linear dichroism and fluorescence spectroscopyJournal of Molecular Biology, 1989
- Structure of helical RecA-DNA complexes: III. The structural polarity of RecA filaments and functional polarity in the RecA-mediated strand exchange reactionJournal of Molecular Biology, 1988
- ENZYMES OF GENERAL RECOMBINATIONAnnual Review of Biochemistry, 1987
- Structure of helical RecA-DNA complexesJournal of Molecular Biology, 1986
- Role of RecA protein spiral filaments in genetic recombinationNature, 1984
- Studies of the interaction of RecA protein with DNANucleic Acids Research, 1983
- Characterization of complexes between recA protein and duplex DNA by electron microscopyJournal of Molecular Biology, 1982
- Elongation of duplex DNA by recA proteinJournal of Molecular Biology, 1981
- Recombination activities of E. coli RecA proteinCell, 1981
- Diffraction by helical structuresActa Crystallographica, 1958