A reappraisal of the complement fixation test using soluble Mycobacterium avium antigen for the detection of M. paratuberculosis infection in cattle
- 1 May 1986
- journal article
- research article
- Published by Wiley in Australian Veterinary Journal
- Vol. 63 (5), 133-134
- https://doi.org/10.1111/j.1751-0813.1986.tb02949.x
Abstract
SUMMARY Serums from 263 cattle suspected of having paratuberculosis on the basis of clinical signs, were tested for antibodies to Mycobacterium paratuberculosis with a complement fixation test (CFT) employing a heat extracted, soluble M. avium antigen. Microscopic examination confirmed that 172 (65.4%) clinically affected animals had paratuberculosis, the remainder being disease‐free. The specificity and sensitivity of the CFT was 92.3% and 74.4% respectively. Phenol treatment of serums before testing was compared with no treatment and was found to have no significant effect on the CFT titres. Results obtained are discussed in relation to the cause of false negative and false positive reactions.Keywords
This publication has 5 references indexed in Scilit:
- Evaluation of the cold complement fixation test for diagnosis of ovine brucellosisAustralian Veterinary Journal, 1982
- Isolation of mycobacteria inducing cross-reactions in the complement fixation test for Johne’s diseaseResearch in Veterinary Science, 1981
- CULTURE OF MYCOBACTERIUM PARATUBERCULOSIS FROM BOVINE FOETUSESAustralian Veterinary Journal, 1979
- JOHNE'S DISEASE WITH PARTICULAR REFERENCE TO DIAGNOSIS. (b) LABORATORY ASPECTSAustralian Veterinary Journal, 1959