Location of the structural genes for glycoproteins gD and gE and for other polypeptides in the S component of herpes simplex virus type 1 DNA

Abstract

To map the structural genes for the gD and gE polypeptides and for other viral products encoded in the S component of herpes simplex virus type 1 DNA, mRNA capable of hybridizing to cloned viral DNA fragments were selected and the mRNA were translated in vitro to determine which polypeptides were encoded therein. The gD and gE polypeptides were identified by immunoprecipitation with appropriate monoclonal and monospecific antibodies; the other polypeptides were characterized only by their electrophoretic mobilities in polyacrylamide gels. gD mRNA hybridized to a single SacI subfragment of BamHI fragment J; gE mRNA hybridized to an adjacent SacI subfragment of BamHI fragment J and also to BamHI fragment X. The structural gene for gD apparently is located between map coordinates 0.911 and 0.924, and the gene for gE is between map coordinates 0.924 and 0.951. mRNA for polypeptides of 55,000, 42,000, 33,000 and 22,000 MW hybridized to DNA fragments spanning the regions from map coordinates 0.911-0.924, 0.897-0.911, 0.939-0.965 and 0.939-0.965, respectively. mRNA for a 68,000 MW polypeptide hybridized to the 2 noncontiguous BamHI fragements N and Z, which share a reiterated DNA sequence.