Genomic structure and chromosomal localization of processed pseudogenes for human RBP-Jk

Abstract

The functional gene for human recombination signal sequence-binding protein (RBP-J_k) and corresponding processed psudogenes have been isolated from various species, such asDrosophila, Xenopus, mouse, and human. Here we report the isolation of another two genomic pseudogenes of human RBP-J_k, named K2 and K7, from a cosmid library of Hela cells. The nucleotide sequences of both genes exhibited more than 95% homology to the functional human gene for RBP-J_k. Moreover, they did not contain any intron sequences and were interrupted by several stop codons in all frames.In situ hybridization demonstrated that the pseudogenes, K2 and K7, were localized at chromosomes 9p13 and 9q13, respectively. Their physical maps differed from those of the true functional gene and of the pseudogenes reported previously by Amakawaet al. (1993).