The new antibiotic, moenomycin, isolated from Streptomyces bambergiensis is a mixture of several, chemically very similar components, which are particularly characterized by their phosphorus content. The moenomycin complex can be separated into components A, B1, B2 and C by means of column chromatography on silica gel with n-propanol - 2N ammonia as eluant. The use of other chromatographic procedures, e. g. chromatography on anion exchange resins, results in a further separation of fraction B1 into components D, E and F ; similarly fraction B2 can be separated into components G and H. Isolation and properties of moenomycins D, E, F, G and H are described.