Antibodies of predetermined specificity against chemically synthesized peptides of human interleukin 2.

Abstract

The preparation of antibodies to human interleukin 2 (IL-2), using as immunogens synthetic peptides derived from the predicted amino acid sequence of IL-2 is described. Rabbits and mice were immunized with protein carrier conjugates of 8 chemically synthesized IL-2-derived peptides, each consisting of 13-15 amino acids. The immune antisera were screened in a solid-phase ELISA [enzyme-linked immunosorbent assay] for reactivity to a native human IL-2. Antibodies to 4 of the 8 peptides were found by a variety of biological and immunochemical criteria to react against human IL-2. An affinity-purified antibody to 1 of the IL-2 peptides (peptide 84) specifically stained the cytoplasm of phytohemagglutinin-stimulated human peripheral blood lymphocytes or T-leukemic cells (Jurkat). Antibodies to synthetic IL-2 peptides should serve as useful probes for studying this lymphokine and for developing quantitative assays for measuring its levels in biological fluids and its association with disease.