Symptomatological, Serological, and Electrophoretic Diversity of Isolates of Andean Potato Latent Virus from Different Regions of the Andes

Abstract

The serological and electrophoretic properties of 26 new isolates of Andean potato latent virus (APLV) from different regions of the Andes varied considerably. Fourteen of these isolates were studied symptomatologically and they all differed. Three major serological strain groups were recognized: the Hu-group was found only in the southern Andes, the CCC-group was found only in the northern Andes, and the Col-Caj-group occurred throughout Andean region, but it was more common in the north than in the south. Serologically, the Hu-group was related more closely to the type and Abelia latent strains of eggplant mosaic virus than to the Col-Caj and CCC-groups. The 3 major strain groups were easily differentiated by enzyme-linked immunosorbent assay, whereas in the latex test all were detected by the same sensitized antiserum. In the agar gel double diffusion test, different antisera differed in ability to differentiate between closely related isolates of the Col-Caj-group.