Localization on pig chromosome 6 of markers GPI, APOE, and ENO1, carried by human chromosomes 1 and 19, using in situ hybridization
- 1 January 1990
- journal article
- research article
- Published by S. Karger AG in Cytogenetic and Genome Research
- Vol. 54 (1-2), 86-91
- https://doi.org/10.1159/000132966
Abstract
In the pig, the linkage group around the halothane gene (HAL), composed of S-GPI-HAL-H-A1BG-PGD, has been assigned to bands p1.2→q2.2 of chromosome 6. In man, ENOl-PGD and APOE-GPI constitute two syntenic groups situated on different chromosomes (1 and 19, respectively). Since GPI and PGD are linked in the pig, we have hybridized the human cDNA probes for ENO1and APOE to pig chromosomes. These markers were assigned to pig chromosome 6, in the q2.2→q2.4 and cen→q2.1 regions, respectively, using in situ hybridization. Since GPI and APOE are situated in the same region, we combined the use of high resolution chromosome analysis and in situ hybridization to give a more precise localization in the q1.2 and q1.2→q2.1.2 regions of chromosome 6. A possible linear order of these genes is proposed.Keywords
This publication has 13 references indexed in Scilit:
- Gene order and recombination rates in the linkage group S-Phi-Hal-H-(Po2)-Pgd in pigsAnimal Blood Groups and Biochemical Genetics, 2009
- THE HIGH-RESOLUTION R-BANDED KARYOTYPE OF THE DOMESTIC PIG, SUS-SCROFA-DOMESTICA L1987
- Molecular cloning and nucleotide sequence of a full-length cDNA for human alpha enolase.Proceedings of the National Academy of Sciences, 1986
- Regional mapping of human chromosome 19: organization of genes for plasma lipid transport (APOC1, -C2, and -E and LDLR) and the genes C3, PEPD, and GPI.Proceedings of the National Academy of Sciences, 1986
- Prediction of the halothane (Hal) genotypes of pigs by deducing Hal, Phi, Po2, Pgd haplotypes of parents and offspring: results from a large‐scale practice in Swedish breedsAnimal Blood Groups and Biochemical Genetics, 1985
- New classes of common fragile sites induced by 5-azacytidine and bromodeoxyuridineHuman Genetics, 1985
- Synthesis, intracellular processing, and signal peptide of human apolipoprotein E.Journal of Biological Chemistry, 1984
- A technique for radiolabeling DNA restriction endonuclease fragments to high specific activityAnalytical Biochemistry, 1983
- Etude du groupe de liaison Hal, Phi et Pgd chez le Porc: disposition relative des trois locus et estimation des taux de recombinaisonGenetics Selection Evolution, 1983
- Quantitative analysis of high-resolution trypsin-Giemsa bands on human prometaphase chromosomesHuman Genetics, 1978