Caffeine enhances fragile (X) expression in somatic cell hybrids
- 1 January 1986
- journal article
- research article
- Published by Wiley in American Journal of Medical Genetics
- Vol. 23 (1-2), 445-455
- https://doi.org/10.1002/ajmg.1320230136
Abstract
Yunis and Soreng recently demonstrated enhanced expression of common fragile sites as and of fra(X) when 2.2 mM caffeine is added to FUdR treated lymphocyte cultures 6 hours before harvest. We failed to replicate this finding for fra(X) expression in we do find a consistent increase in expression levels in somatic cell hybrids between a Chinese hamster cell line and 3 unrelated individuals with the fra(X) mutation when caffeine is present for the last 2 hours before harvest. This was particularly true for “low-expressing” cell lines, in which a 4-6 fold increase was observed. Using a thymidylate synthase deficient hybrid which could be blocked in the S phase by thymidine deprivation, we found that caffeine significantly reduced the recovery time from thymidine release to mitosis. This produced the highest level of fra(X) expression (48%) only one hour after release from thymidine deprivation. These results show that caffeine does enhance fra(X) expression in at least some cell types. The effect is probably, inhibiting the mitotic delay usually associated with DNA damage.Keywords
This publication has 11 references indexed in Scilit:
- Somatic cell hybrid studies of fragile (X) expression in a carrier female and transmitting maleAmerican Journal of Medical Genetics, 1986
- Effect of caffeine and adenosine on G2 repair: mitotic delay and chromosome damageMutation Research, 1985
- Constitutive Fragile Sites and CancerScience, 1984
- Expression of the fragile (X) chromosome in an interspecific somatic cell hybridHuman Genetics, 1983
- Marker X syndrome in an oriental family with probable transmission by a normal maleAmerican Journal of Medical Genetics, 1982
- Mechanism by which caffeine potentiates lethality of nitrogen mustard.Proceedings of the National Academy of Sciences, 1982
- Radiosensitivity in ataxia-telangiectasia: a new explanation.Proceedings of the National Academy of Sciences, 1980
- Absence of a measurable G2 phase in two Chinese hamster cell lines.Proceedings of the National Academy of Sciences, 1977
- CaffeineMutation Research/Reviews in Genetic Toxicology, 1977
- The enhancement by caffeine of alkylation-induced cell death, mutations and chromosomal aberrations in Chinese hamster cells, as a result of inhibition of post-replication DNA repairMutation Research, 1974