Localization of the thermosensitive X‐prolyl dipeptidyl aminopeptidase in the vacuolar membrane of Saccharomyces cerevisiae
- 23 July 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 173 (1), 199-203
- https://doi.org/10.1016/0014-5793(84)81046-7
Abstract
Most of the X-prolyl dipeptidyl aminopeptidase activity of Saccharomyces cerevisiae was found to be associated with purified vacuolar membranes (specific activity approx. 75-times higher than in the protoplast lysate). The tonoplast-bound enzyme is thermosensitive. Another heat-resistant enzyme was found in the protoplast lysate. The tonoplast-bound thermosensitive enzyme shows an apparent K m of 0.06 mM against L-alanyl-L-prolyl-p-nitroanilide while the heat-resistant enzyme shows an apparent K m of 0.4 mM against the same substrate.Keywords
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