Ion fluxes and electrical characteristics of the short-circuited rat colon in vitro
- 1 July 1982
- journal article
- research article
- Published by Springer Nature in Pflügers Archiv - European Journal of Physiology
- Vol. 394 (1), 32-37
- https://doi.org/10.1007/bf01108305
Abstract
The unidirectional fluxes of sodium and chloride across stripped rat colon mucosa were measured and compared with the electrical characteristics of the tissue under voltage-clamped conditions. The relationship between the serosal-mucosal fluxes of the two ions and an imposed potential revealed that the serosalmucosal flux of sodium was entirely paracellular, whereas there was also a transcellular component of the corresponding chloride flux. In the absence of sodium, the short-circuit current and net chloride flux were abolished; in the absence of chloride, the net sodium flux was reduced but not abolished, and the shortcircuit current was unchanged. From an analysis of the effects of the inhibitors, amiloride, theophylline, acetazolamide, furosemide and piretanide, a plausible model was developed to explain the characteristics of these transports. It was proposed that both membranes posses Cl−/HCO 3 − antiports, though their sensitivities to inhibitors were different. There is also a Na+/Cl− symport and an electrogenic sodium entry mechanism in the brushborder membrane.This publication has 26 references indexed in Scilit:
- The short-circuited everted sac of rat colon mucosaZeitschrift für Die Gesamte Experimentelle Medizin, 1981
- Effects of bicarbonate on fluid and electrolyte transport by the guinea pig gallbladder: A bicarbonate-chloride exchangeThe Journal of Membrane Biology, 1979
- Inhibition of active chloride transport by piretanideNature, 1978
- Segmental heterogeneity of epithelial transport in rat large intestinePflügers Archiv - European Journal of Physiology, 1978
- Ion transport by rabbit colonThe Journal of Membrane Biology, 1976
- Inhibition of Transport Processes in the Dog ColonPublished by Springer Nature ,1976
- Transport of amino-acids and sugars by the dog colonic mucosaPflügers Archiv - European Journal of Physiology, 1973
- Ionic Conductances of Extracellular Shunt Pathway in Rabbit IleumThe Journal of general physiology, 1972
- Bicarbonate Secretion by the Rat Colon: Effect of Intraluminal Chloride and AcetazolamideExperimental Biology and Medicine, 1970
- FLUID AND SOLUTE TRANSPORT ACROSS RAT COLONIC MUCOSAQuarterly Journal of Experimental Physiology and Cognate Medical Sciences, 1965