Rabbit Antiserum Against a Non-T, Non-B Leukemia Cell Line That Carries the Ph1 Chromosome (NALM-1): Antibody Specific to a Non-T, Non-B Acute Lymphoblastic Leukemia Antigen2

Abstract

Rabbit immune sera against human cells of a non- T, non-B leukemia cell line bearing the Ph¹ chromosome (NALM- 1) were characterized. After proper and exhaustive absorption, the sera were tested by indirect membrane immunofluorescence on a panel of 19 human hematopoietic cell lines with T-, B-, or non-T, non-B cell surface characteristics. The absorbed sera reacted specifically with NALM-1 cells but not with cells of another Ph¹-positive cell line, K-562. Four of the 6 leukemia T-cell lines, 2 of the 4 leukemia-lymphoma B-cell lines, all of 4 acute lymphoblastic leukemia (ALL) lines with non-T, non-B cell characteristics, and uncultured cells of all patients with non-T, non-B cell ALL were reactive with the antisera. A cross-absorption study of the antisera suggested that a single antigenic complex is involved in this antigen specificity. The antigen involved appears to be a common non-T, non-B ALL one that has been described previously.