Essential structure ofE. colipromoter effect of spacer length between the two consensus sequences on promoter function
Open Access
- 1 January 1983
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (17), 5855-5864
- https://doi.org/10.1093/nar/11.17.5855
Abstract
A promoter with the consensus sequences(TTGACA and TATAAT) at −35 and −10 regions was constructed, and the distance between the two consensus sequences was independently altered at two restriction sites. In an in vitro transcription system, a maximum activity was observed at the spacer length of 17 base-pairs regardless of the sites of space adjustment. Evidence was also provided that the start point of transcription was fixed by the consensus sequence at the −10 region.Keywords
This publication has 15 references indexed in Scilit:
- Compilation and analysis ofEscherichia colipromoter DNA sequencesNucleic Acids Research, 1983
- A rapid boiling method for the preparation of bacterial plasmidsAnalytical Biochemistry, 1981
- Organization of transcriptional signals in plasmids pBR322 and pACYC184.Proceedings of the National Academy of Sciences, 1981
- E. coli RNA polymerase interacts homologously with two different promotersCell, 1980
- REGULATORY SEQUENCES INVOLVED IN THE PROMOTION AND TERMINATION OF RNA TRANSCRIPTIONAnnual Review of Genetics, 1979
- The nucleotide sequence of the initiation and termination sites for ribosomal rna transcription in x. laevisCell, 1979
- Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybridsCell, 1977
- Interaction of RNA Polymerase with Promoters from Bacteriophage fdEuropean Journal of Biochemistry, 1977
- A new method for sequencing DNA.Proceedings of the National Academy of Sciences, 1977
- A dye-buoyant-density method for the detection and isolation of closed circular duplex DNA: the closed circular DNA in HeLa cells.Proceedings of the National Academy of Sciences, 1967