Preliminary biochemical characterization of the factors(s) responsible for herpesvirus-induced exogenous fusion
- 1 June 1976
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 18 (3), 1081-1087
- https://doi.org/10.1128/jvi.18.3.1081-1087.1976
Abstract
Cell-free extracts prepared from herpes simplex virus[HSV]-infected BHK-21 [baby hamster kidney] cells rapidly induced exogenous fusion when incubated with indicator monolayers of uninfected BHK-21 cells. Fusion was first observed at 1 h, and peak activity was reached by 4 h. Divalent cations were required for activity. Inhibition of indicator cell macromolecular synthesis with metabolic inhibitors [cycloheximide, actinomycin D, mitomycin C and cytosine arabinoside] failed to prevent formation of cell-free extract-induced polykaryocytes. Removal of virus particles from the cell-free extract by velocity sedimentation centrifugation did not affect cell-free extract exogenous fusion activity. Studies using molecular probes, i.e., glycosidases, lectins and antiserum (directed against HSV envelope or capsid proteins), suggest that the factor(s) responsible for herpesvirus fusion is a fucosylated glycoprotein that is not a structural component of the virion.This publication has 21 references indexed in Scilit:
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