Nitraminoacetic acid (C2H4N2O4) which showed partial inhibition of some gram-negative bacteria was produced by Streptomyces noursei 8054-MC3. It is extracted from culture filtrate with butanol and after chromatographic purification it is recrystallized from mixed solvent of ethylacetate and chloro form as colorless needles. It is acidic and melts at 106°C. Physicochemical properties including n.m.r., infrared and ultraviolet spectra, and its chemical synthesis are also reported.