Silica gel: an improved support for the solid-phase phospphotriester synthesis of oligonucleotides
Open Access
- 25 November 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 10 (22), 7439-7448
- https://doi.org/10.1093/nar/10.22.7439
Abstract
The phosphotriester method for the stepwise synthesis of deoxyoligonucleotides has been employed using HPLC-grade silica gel (Porasil B) as the solid support. The procedure results in a convenient flow-through system for the synthesis of oligomers where all the reaction steps including the zinc bromide method of detritylation are compatible with the selected support. Deoxyoligonucleotides of 25-30 nucleotides in length can be synthesized in high yields utilising stable phosphotriester intermediates. Ease of handling of the solid support allows convenient synthesis of mixed oligonucleotide sequences.Keywords
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