COMBINED STAINING OF CORNEAL ENDOTHELIUM BY ALIZARINE RED AND TRYPANE BLUE

Abstract

Combined staining by alizarin red and trypan blue was an easy and reliable method for in vitro visualization of corneal endothelial nuclei, cellular borders and uncovered parts of the membrane of Descemet. A stained full thickness wet mount could be prepared in less than 5 min. The staining method [developed for evaluation of corneas for transplantation] and preliminary findings concerning endothelial morphology in the center and in the periphery of normal corneas [bovine and human] were presented.