Inositol cyclic triphosphate [inositol 1,2-(cyclic)-4,5-triphosphate] is formed upon thrombin stimulation of human platelets.
- 1 September 1986
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 83 (17), 6397-6401
- https://doi.org/10.1073/pnas.83.17.6397
Abstract
Cleavage of polyphosphoinositides in vitro by phospholipase C results in formation of both cyclic and noncyclic phosphates. We have now isolated the cyclic product of phosphatidylinositol 4,5-bisphosphate cleavage, inositol 1,2-(cyclic)-4,5-trisphosphate [cIns(1:2,4,5)P3], from thrombin-treated platelets. We found 0.2-0.4 nmol of cIns(1:2,4,5)P3 per 109 platelets at 10 sec after thrombin; none was found in unstimulated platelets or in platelets 10 min after thrombin addition. We conclude that cIns(1:2,4,5)P3 is a major product of polyphosphoinositide metabolism in thrombin-stimulated platelets.This publication has 20 references indexed in Scilit:
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