Poliovirus type 3: molecular cloning of the genome and nucleotide sequence of the region encoding the protease and polymerase proteins
Open Access
- 11 March 1983
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 11 (5), 1267-1281
- https://doi.org/10.1093/nar/11.5.1267
Abstract
Overlapping cDNA clones representing the entire genone of poliovirus type 3 have been prepared in E. coli by two separate methods. Cloning of RNA.cDNA hybrids produced a more comprehensive set of clones with generally larger cDNA inserts than cloning of double - stranded cDNA. A restriction map of the entire genome and the nucleotide sequence of 2003 bases from the 3′ terminus, comprising the region encoding the protease and polymerase proteins, are presented.Keywords
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