Expression of human mammary epithelial (HME) antigens in primary cultures of normal and abnormal breast tissue
- 15 December 1978
- journal article
- research article
- Published by Wiley in International Journal of Cancer
- Vol. 22 (6), 655-661
- https://doi.org/10.1002/ijc.2910220603
Abstract
The expression of human mammary epithelial (HME) antigens was studied in primary cell cultures of normal and abnormal breast tissue and of tissues from other organs. By indirect immunofluorescence it was shown that heterologous anti‐HME bound to the cell surface of both suspension and monolayer cultures of human breast epithelial cells from normal breast tissue, normal breast fluid, non‐malignant atypical lesions of the female breast, gynecomastia in the male breast, breast carcinomas, and peritoneal and pleural effusions of patients with breast carcinomas. The fibroblast‐like cells cultured from either normal, non‐malignant atypical, or malignant breast tissue never bound anti‐HME, nor did histiocyte‐like cells from breast fluid or a sarcoid granuloma of the breast. From a Paget tumor of the breast, two distinguishable epithelial‐like colonies appeared in the cultures, only one of which expressed HME‐antigens. Anti‐HME did not bind to cells cultured from tissues of other organs, whether epithelial‐like or fibroblast‐like; these organs included thyroid, ovary, endometrium, and skin.This publication has 22 references indexed in Scilit:
- Effects of sodium butyrate on synthesis of human chorionic gonadotrophin in trophoblastic and non-trophoblastic tumoursNature, 1977
- Surface differentiation antigens of human mammary epithelial cells carried on the human milk fat globule.Proceedings of the National Academy of Sciences, 1977
- Secretion of hCG-α subunit and hCG by HeLa strainsNature, 1977
- HeLa cells secrete α subunit of glycoprotein tropic hormonesNature, 1976
- Hormone induction of specific protein synthesis in midpregnant mouse mammary cell cultureJournal of Experimental Zoology, 1976
- Production of human chorionic gonadotropin in HeLa cell culturesNature, 1976
- Human α-Lactalbumin: Measurement in Serum and in Breast Cancer Organ Cultures by RadioimmunoassayScience, 1975
- Cellular Receptors and Mechanisms of Action of Steroid HormonesInternational Review of Cytology, 1975
- Human Breast Tumor Cell Lines: Identity Evaluation by Ultrastructure 2JNCI Journal of the National Cancer Institute, 1974
- Deletion of liver-cell surface membrane components from aminoazo-dye-induced rat hepatomasInternational Journal of Cancer, 1972