Chemical Composition of Cell-wall Polysaccharide of Rough Mutants of Salmonella typhimurium

Abstract

SUMMARY: Quantitative analyses of monosaccharide constituents of the cell-wall polysaccharide of the smooth form and rough mutants of Salmonella typhimurium were made by gas-liquid chromatography of glycitol acetates produced from acid hydrolysates of phenol+water extracts of cell-wall preparations. The presence, in varying amounts, of sugars characteristic of the S-specific repeating unit was detected in all rough mutants investigated. Definite conclusions about the core structure of the different rough mutants could not be drawn by using only the basis of the monosaccharide composition of the cell-wall polysaccharide. Ribose was found in the cell-wall polysaccharide of the smooth form in small amounts.