‘Cap 90’, a 90‐kDa Ca2+‐dependent F‐actin‐capping protein from vertebrate brain
- 14 November 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 163 (2), 225-229
- https://doi.org/10.1016/0014-5793(83)80824-2
Abstract
A Ca2+‐dependent actin filament‐capping protein of 90 kDa was purified from bovine brain using a new and rapid isolation procedure. This basically includes affinity purification on DNase‐I agarose. The protein caps the fast‐growing end of actin filaments but has no fragmenting or severing activity. Using Triton X‐100‐extracted cytoskeletons, capping and severing activities of actin‐binding proteins become clearly distinguishable from each other.Keywords
This publication has 19 references indexed in Scilit:
- Disruption of microfilament organization after injection of F-actin capping proteins into living tissue culture cellsNature, 1983
- Three-dimensional structure of actin filaments and of an actin gel made with actin-binding protein.The Journal of cell biology, 1983
- Actin-binding proteins—regulators of cell architecture and motilityNature, 1982
- Actin-binding proteinsTrends in Biochemical Sciences, 1982
- An actin-binding protein from Acanthamoeba regulates actin filament polymerization and interactionsNature, 1980
- Identification of a factor in conventional muscle actin preparations which inhibits actin filament self-associationBiochemical and Biophysical Research Communications, 1980
- Viscometric analysis of the gelation of Acanthamoeba extracts and purification of two gelation factors.The Journal of cell biology, 1980
- A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye bindingAnalytical Biochemistry, 1976
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970
- Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gelsBiochemical and Biophysical Research Communications, 1967