Isolation and sequence analysis of a cDNA clone encoding the entire catalytic subunit of phosphorylase kinase
- 10 August 1987
- journal article
- Published by Wiley in FEBS Letters
- Vol. 220 (1), 36-42
- https://doi.org/10.1016/0014-5793(87)80871-2
Abstract
Synthetic oligonucleotides have been used to isolate a 1.85 kb clone containing the full length coding sequence for the catalytic subunit of rabbit skeletal muscle phosphorylase kinase from a cDNA library constructed in λgt10. Sequence analysis of the clone predicted an amino acid sequence in agreement with a published primary structure. Inspection of the codon usage revealed a strong preference for G or C nucleotides at the third codon position as found for several other skeletal muscle proteins. This cDNA clone should facilitate identification of functional domains, including the calmodulin-binding site, and investigation of the molecular basis of X-linked phosphorylase kinase deficienciesKeywords
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