Similarities in nucleotide sequence between serum and faecal human parvovirus DNA

Abstract

SUMMARY: Two DNA clones were obtained from faecal specimens containing a parvovirus-like small round virus from a 1977 outbreak of gastroenteritis, and their nucleotide sequences were determined and found to be essentially identical with parts of the published sequence of serum parvovirus B19 and with a B19 isolate (JB) partially sequenced in this study. The clones corresponded mainly to genome regions coding for non-structural proteins, but also include a sequence of some 160 bp coding for structural proteins. Southern blotting experiments with a full-length B19 probe revealed a virion-sized 5·5 kbp DNA band in specimens from gastroenteritis cases in both 1977 and 1986. Thus the nucleotide sequence and hybridization results suggest that the virus seen in these studies is very similar to B19. Further work is necessary to clarify the antigenic relationship of these viruses.