The potato eelworm hatching factor. 3. Concentration of the factor by chromatography. Observations on the nature of eclepic acid
- 1 January 1949
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 45 (5), 524-528
- https://doi.org/10.1042/bj0450524
Abstract
The application of 2-phase chromatography on silica gel loaded with strong phosphate buffer to the concentration of the potato eelworm hatching factor is descr. Chromatography at pH 6 followed by brucine salt fractionation. or 2- stage chromatography at pH 5.5-6 and 4-4.5, effected similar degrees of concentration, the resinous products being fully active in hatching eelworm cysts at dilutions of 1 in 10''. Eclepic acid contains a lactone and hydroxyl group.Keywords
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