Comparison of histological and immunological techniques for detection of Pneumocystis carinii in rat bronchial lavage fluid
- 1 April 1980
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 11 (4), 409-417
- https://doi.org/10.1128/jcm.11.4.409-417.1980
Abstract
One compared histological and immunological techniques in the early diagnosis of P. carinii pneumonia in bronchial lavage fluid of steroid-treated rats. The rats were sacrificed weekly and lavage fluids were examined with cresyl echt violet and Giemsa stains; examined for P. carinii antigens by indirect fluorescent-antibody, counterimmunoelectrophoresis and double-diffusion techniques, using high-titer specific antisera to P. carinii raised in rabbits. P. carinii was detected in lavage fluid by cresyl echt violet at 2 wk of steroids and persisted even with steroid tapering; the intensity of the infection in lavage fluid closely paralleled that in the lungs. P. carinii was not detected in lavage by Giemsa stain until 4 wk and disappeared from the fluids with steroid tapering. P. carinii was detected by indirect fluorescent antibody as early as 1 wk of steroids and the results correlated well with those of cresyl echt violet. P. carinii antigens were not detected in lavage fluids or serum by counterimmunoelectrophoresis or double-diffusion techniques. Although precipitin lines sometimes occurred, they were nonspecific. In this model, cresyl echt violet and indirect fluorescent antibody were the preferred techniques for the early diagnosis of P. carinii infection in bronchial lavage fluid.This publication has 36 references indexed in Scilit:
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