Alkaline phosphatase activity does not mediate phosphate transport in the renal-cortical brush-border membrane
- 15 August 1980
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 190 (2), 473-476
- https://doi.org/10.1042/bj1900473
Abstract
The effect of primary modulators of phosphate transport, namely the hypophosphatemic mouse mutant (Hyp) and low-P diet, on alkaline phosphatase activity in mouse renal-cortex brush-border membrane vesicles and the effect of several primary inhibitors of alkaline phosphatase on phosphate transport was studied. Brush-border membrane vesicles from Hyp-mouse kidney had a 50% loss of Na+-dependent phosphate transport and an 18% decrease in alkaline phosphatase activity. The low-P diet effectively stimulated Na+/phosphate co-transport in brush-border membrane vesicles (+ 118%), but increased alkaline phosphatase activity only slightly (+ 13%). Levamisole (0.1 mM) and EDTA (1.0 mM) inhibited brush-border membrane-vesicle alkaline phosphatase activity by 82% and 93%, respectively, but had no significant effect on Na+/phosphate co-transport. Apparently alkaline phosphatase does not play a direct role in phosphate transport across the brush-border membrane of mouse kidney.This publication has 17 references indexed in Scilit:
- Renal handling of phosphate in vivo and in vitro by the X-linked hypophosphatemic male mouse: Evidence for a defect in the brush border membraneKidney International, 1978
- The defect in transcellular transport of phosphate in the nephron is located in brush-border membranes in X-linked hypophosphatemia (Hyp mouse model)Canadian Journal of Biochemistry, 1978
- Microvilli: their ultrastructure, enzymology and molecular organization.1978
- Hypophosphatemia: mouse model for human familial hypophosphatemic (vitamin D-resistant) rickets.Proceedings of the National Academy of Sciences, 1976
- Phenylalanine uptake in isolated renal brush border vesiclesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1976
- The effect of several diphosphonates on acid phosphohydrolases and other lysosomal enzymesBiochimica et Biophysica Acta (BBA) - Enzymology, 1976
- A rapid method for the preparation of microvilli from rabbit kidneyBiochemical Journal, 1974
- Studies on the enzymology of purified preparations of brush border from rabbit kidneyBiochemical Journal, 1973
- Kinetics and inhibition of alkaline phosphatases from canine tissuesBiochimica et Biophysica Acta (BBA) - Enzymology, 1972
- Phosphate absorption and alkaline phosphatase activity in the small intestine of the adult mouse and of the chick embryo and hatched chickComparative Biochemistry and Physiology Part A: Physiology, 1972