Transition from Cometabolic to Growth-Linked Biodegradation of Vinyl Chloride by a Pseudomonas sp. Isolated on Ethene

Abstract

Pseudomonas aeruginosa strain DL1 was isolated on ethene as a sole carbon and energy source. When ethene-grown DL1 was first exposed to vinyl chloride (VC), the rate of VC consumption was very rapid and then declined sharply, indicative of a cometabolic process. A lack of growth and significant release of soluble products during this interval also indicates that the initial activity on VC was cometabolic. Following the rapid initial rate of VC cometabolism, a slow rate of VC utilization continued. After an extended period of incubation (>40 days), a transition occurred that allowed DL1 to begin using VC as a primary growth substrate, with an observed yield, maximum growth rate, and Monod half saturation coefficient of 0.21 mg of total suspended solids/mg VC, 0.046 d(-1), and 1.17 microM VC, respectively, at 22 degrees C. Acetylene inhibits consumption of ethene and VC by ethene-grown cells, suggesting a monooxygenase is responsible for initiating metabolism of these alkenes. Resting cells grown on ethene cometabolized VC with an observed transformation capacity of 9.1 micromol VC/mg total suspended solids and a transformation yield of 0.22 mol VC/mol ethene. The presence of 40 microM ethene increased the rate and amount of VC cometabolized. However, consumption of higher concentrations of ethene decreased the total amount of VC consumed, and VC inhibited ethene utilization. A kinetic model was developed that describes substrate interactions during batch depletion of ethene and VC for a range of initial concentrations. The results suggest that ethene may stimulate in situ biodegradation of VC either by functioning as a primary substrate to support cometabolism of VC or by selecting for organisms that can utilize VC as a primary substrate.