Über Thrombokinase und Prothrombokinase des Blutes

Abstract

C02 rich blood clots more slowly than CO2 poor blood and the prothrombokinase may be converted into the active thrombokinase by a sudden fall of the CO2 tension. Oxalated or citrated human plasma is diluted 1:1 with H2O and a brisk stream of CO2 passed through the soln. at 38[degree] for 5 min. At various intervals after the CO2 is discontinued, 0.1 ml. samples of the plasma are recalcified with 0.1 ml. of 0.10% CaCl2 and the clotting time of the mixture detd. The plasma untreated with CO2 clots in 124 sec. when re-calcified. The sample of plasma removed 0 min. after treatment with CO2 clots in 141 sec., but as the time interval increases, the clotting time of the treated plasma falls to values which are obtained with the aid of clotting active tissue extracts. After 32 min., the plasma clots in 19 sec., and from then on the clotting time gradually returns to normal in 240 min. The acceleration of the clotting is due to the conversion of prothrombokinase into thrombokinase because of the treatment with CO2.